Figure 6
From: Genome-wide DNA methylation changes in skeletal muscle between young and middle-aged pigs
Examples of age-related genes showing differential DNA methylation in their gene bodies. (A) DMR in FOXO3 gene body. (B) DMR in FGFR1 gene body. (C) DMR in GRB2 gene body. Top panels, Top half: CpG methylation. Each point represents methylation level (MeDIP-seq read depth) of a sample at a given CpG site. The curves showed average over the samples. The two vertical dashed lines marked the boundaries of the DMR identified. Lower half: CpG dinucleotides (black tick marks on X axis), CpG density (grey line). Plus and minus marks denote sense and antisense gene transcription. Second panels: validation of CpG methylation by bisulfite sequencing PCR (BSP). Ten subclones were selected for the BSP analysis. The solid circles represent the methylated CpG sites, and the open circles represent the unmethylated CpG sites. Third panels: comparison of gene expression levels between two age groups. The gene expression levels were taken from microarray data and normalized to the expression levels in young pigs.