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Table 2 Modified emulsion PCR conditions for mate pair library

From: Comparative genomics of 274 Vibrio cholerae genomes reveals mobile functions structuring three niche dimensions

Stage Step Temperature Time
Hold Denature 94°C 6 minutes
Cycle (40) Denature 94°C 30 seconds
Anneal 58°C 30 seconds
Extend 72°C 90 seconds
Cycle (20) Denature 94°C 30 seconds
Extend 58°C 18 minutes
Hold   4°C infinity
  1. The emulsion PCR conditions of the mate pair library construction for Ion Torrent sequencing of V. cholerae strain R-18308.