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Table 2 Modified emulsion PCR conditions for mate pair library

From: Comparative genomics of 274 Vibrio cholerae genomes reveals mobile functions structuring three niche dimensions

Stage

Step

Temperature

Time

Hold

Denature

94°C

6 minutes

Cycle (40)

Denature

94°C

30 seconds

Anneal

58°C

30 seconds

Extend

72°C

90 seconds

Cycle (20)

Denature

94°C

30 seconds

Extend

58°C

18 minutes

Hold

 

4°C

infinity

  1. The emulsion PCR conditions of the mate pair library construction for Ion Torrent sequencing of V. cholerae strain R-18308.