Figure 3

Enrichment profile of the immunologically relevant pathways significantly altered by GoI-LPSμG with a focus on Toll-like receptor (TLR) pathway co-enriched by GoI-LPSμG and GoI-μG. A. Enrichment profile of the immunologically relevant pathways significantly altered in responding to LPS assault mediated by different gravitational forces. Twenty four most significantly enriched pathways were identified focusing on three parent nodes, namely cytokine/chemokine pathways (11 networks) apoptosis pathways (5 networks) and growth factor pathways (8 networks). For each network, there are four bars segregated into two columns (Ground and Space) and two rows (4 h and 8 h, which are noted as 4 and 8, respectively). The distributions of genomic regulations associated with each pathway are hereby documented for G-4 h, G-8 h, S-4 h and S-8 h and the pathways are batched into three parent pathways: cytokine/chemokine pathways, apoptosis pathways and growth factor pathways. The fraction of the elevated (fold change > 2) and suppressed (fold change < −2) transcripts are colored by red and green, respectively. The unchanged fractions are yellow. The color scheme is at the right. B. Toll-like receptors (TLR) pathway. A schematic network of TLRs pathway is presented here. The TLRs pathway is significantly co-enriched by both gene sets linked to LPS insult mediated by microgravity (GoI-LPSμG) and exclusive effects of microgravity (GoI-μG), respectively. There are three blocks beneath each gene name representing the transcriptomic regulations of S-C (left most block), G-4 h (middle block) and S-4 h (right most block). G-8 h and S-8 h are omitted. The S-C regulations normalized by G-C (S-C/G-C) represent the exclusive impacts of microgravity. And, G-4 h and S-4 h normalized by respective controls (G-4 h/G-C and S-4 h/S-C) represent the influence of the gravitational shift on host response to LPS assault. The scale of the color scheme is included.