Table 6 Primer sequences used for validation in qRT-PCR analysis
Name | Blast SOL network genomics | Annotation | Foward sequence | Reverse sequence |
|---|---|---|---|---|
0U17 | NM | Protein | ACTACCAACATTCACAGCAGCTC | TTAACCCTGTTGAAGGTTAGTGC |
0U6 | SGN E1316291 | Acid phosphatase | CTAATTAACCCTCTCCGCATGAT | GCCAACTCAGGCAATTATATACG |
0U14 | SGN E667484 | Protein | TAGTCAAGAATATGGGCATGGAC | ATACCTTCTTGATTCACGCCTTC |
0U2 | SGN E1352064 | Caffeine synthase | AAAGGGAGCATTTACTCTTCCAAAG | AGCATGCATCCTGAGAAATGTGGTA |
0U4 | SGN E1310344 | Metallothionein-like protein | ATTCGTCTGCTCTGTGAAGATGT | ATACATGTTTCCGCAGTTTCCT |
0U322 | SGN E450221 | nadp-dependent d-sorbitol-6-phosphate dehydrogenase | CCTTTGTGGCTTCTAAGCAAAT | GGAAAGCAGAGATTGACAAACAG |
0U1 | SGN E628893 | No hits | CAAGGAAGATGCTTTTGACGAT | TGTAATTATGCTGCTGGTGCTAC |
0U1 | SGN E628893 | No hits | CATTTAGTTTGGAAGGGGACAA | GGATACAGCCGGTAGGACTAACT |
0U42 | SGN E636199 | ---NA--- | ACCCGCCGGGAAACC | GATGCACAGACAGGAATCACAAC |
0D6 | SGN E661762 | ---NA--- | TTGGTAATGATGGAAGTGTCCTC | GCCAGTAATGGGATTGTAGTTGA |
0D10 | SGN E1033676 | Glycerol-phosphate acyltransferase | CTAGCTTGACCAGGAAAGACAAG | GACTCAGGACTGCTCATTTCATT |
0D2 | SGN E676870 | Protein | GGCAACTACTGCATTCTATCAGC | AAATGGATGAGCTGAAGGAGAAC |
0D1 | SGN E1320843 | Protein | ACTAGTACTGGGTGTTGCCTCAA | GGTGAGCAAATAGTTGTTGTTGC |
0D19 | SGN E673783 | Isocitrate lyase | GGCCAGGAGCAACAGACATT | ATTCTCTCACAATCTTGACTTTGCA |
0D3 | SGN E1320843 | Zinc finger | CTGATTACGTCCGCTATCTCATT | AACCTATCGGACCTGTACCTGTT |
0D4 | SGN E1325444 | Hypothetical protein | TACTGGCACTAATGGAGGAAATG | AATGGGGACAGATGTATCATCAC |
0D8 | NM | Glycerol-3-phosphate acyltransferase 6 | TTCAAGAGTTTGGTACTGACGTG | ATCATGGTCTGTCTCTCGATCTC |
0D9 | SGN E1321887 | gdsl-motif lipase hydrolase-like | TCTTAACTGGACTTCCTCCAATG | CCATTGAAGTTTAGAGCCACAAC |
0D7 | NM | Hypothetical protein | CCAAGACAGTTGATCTCCCTCTA | CGTAGTAGCTAGATGGTGCCAGT |
0U9 | SGN-E1337775 | Organ-specific protein | GGTTTCTTTAGGGTTTCCTTCCT | CACAGTGTGTGTGTTTTGTTCCT |
0U11 | SGN-E1352070 | Caffeine synthase | CCTAGCAAGCCATTTTGGAG | ATTCTTGGCAAACCTGTGGA |
0U6 | SGN-E1316291 | Acid phosphatase | ATTACGGCTATGGCAGAATTAGC | CACCATGTTTCCTTGTTTGAGA |
0U3 | SGN-E1326397 | Acidic endochitinase se2 | CAGCAAATTCTTCCCTATGTCC | CAGCGTTTCAGGGTTAACATAAG |
0U8 | SGN-E1327615 | Kunitz trypsin inhibitor | CTCTTCCTTTCATTTCTGCTCTTC | GACGTAGTACTCGACACCAGGAC |