Figure 2

Direct comparison of the transcriptional responses to DEX and PRED exposure. A: Volcano plot showing the difference in GC regulation (M, i.e. log2 fold-change) for each gene against its significance (-log10 of the p-values adjusted for multiple hypothesis testing) between 6 hours prednisolone (PRED) or dexamethasone (DEX) treatments. The grey-dashed horizontal line represents the significance cut-off (5% FDR), the dashed vertical lines the cut-off for the extent of regulation (two-fold difference). Points are colored according to the local point density. B: Correlation of M-values representing the extent of gene regulation by DEX or PRED treatment. Shown are genes identified to be significantly regulated by either DEX or PRED treatment. The grey-dashed and the solid black line represent the identity line and the linear regression fit to the data, respectively.