Differentiation of the atp 6 alleles revealed by amplification using two sets of forward primers designed based on mitochondrial genome sequences (Additional file
: Table S3). The atp6-T amplification of atp 6 allele from the T. turgidum and atp6-L amplification of atp 6 from the Aegilops longissima and the (lo) durum line. DNA was separated on a 1% agarose gel. M indicates bp size marker, C indicates control PCR, PD - primers dimers.