Figure 3

Negative correlation between TIN - estimates and splicing factor expression in the cancer datasets. In the seven cancer datasets (grey background) with strong associations between TIN-estimates and expression levels of splicing factors (n = 280), the relationship was inverse, with a much higher percentage of significant negatively (horizontal axes) than positively (vertical axes) correlated splicing factor genes (red). This shift was higher than expected by chance, as demonstrated by comparisons with genes in each of 1,000 random sets of 280 genes (light blue), and with each of 1,000 permutations of the TIN-estimates (dark blue). This significant shift towards negative correlation was not found in the three cancer datasets with weak associations between TIN-estimates and splicing factor expression (no background).