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Table 2 Summary of DNA biophysical characterization of representative 2G QFP and corresponding control sequences

From: Association of G-quadruplex forming sequences with human mtDNA deletion breakpoints

   

Min. distance

Melting T1/2°C*

Oligo

Sequence

Midpoint

5′

3′

A

GGA TGG GGT GGG GAG G

14813

27

0

80.0±1.5

Ac

AGA TGG AGT GGA GAG G

   

11.6±1.0

B

GGG GGA TGC GGG GG

13760

1

0

85.2±0.9

Bc

AGG AGA TGC AGG AG

   

14.0±2.0

C

GGA GGG TGG ATG G

5206

11

0

53.6±1.0

Cc

AGA GGG TAG ATG G

   

26.9±0.5

  1. Oligonucleotides with QFP (A-C) or control sequences as indicted with (c) (mutations to ablate QFP are shown in bold) were tested for folding stability as reflected through their melting temperature, T1/2. Midpoints of these sequences, their minimum distance to a 5′ and 3′ breakpoint, and T1/2 are indicated. Melting temperatures were determined from CD melting curves as described in Methods section.