Table 1 Long-range PCR primers for amplifying the complete human mitochondrial genome
Primer name | Sequence (5′–3′) | Length (nt) | 5′–3′ binding position in revised Cambridge Reference Sequence (rCRS) | Expected product size (bp) |
|---|---|---|---|---|
HumLR_1F | ACGGGAAACAGCAGTGATTAAC | 22 | 807–828 | 8511 |
HumLR_1R | CTAGTATGAGGAGCGTTATGGAGTG | 25 | 9342–9318 | |
HumLR_2F | GTACGCCTAACCGCTAACATTACT | 24 | 8998–9021 | 8735 |
HumLR_2R | GTTTTAAGCTGTGGCTCGTAGTG | 23 | 1163–1141 |