Table 6 Real-time PCR primer pairs for the expression analyses of PAL , CHS , F3H , and IFS genes
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR product length (bp) |
|---|---|---|---|
Actin4 | GTGTCAGCCATACTGTCCCCATTT | GTTTCAAGCTCTTGCTCGTAATCA | 214 |
PAL | ATTATGGATTCAAGGGAGCT | AATGAGGAAAGTGGAGGACA | 182 |
CHS | AAAATGCCATCTCCTCAAACA | GGATCTCAGCTACGCTCACC | 155 |
F3H | GCTTGCGAGAATTGGGGTAT | CCTTGGAGATGGCTGGAGAC | 176 |
IFS | GCCCTGGAGTCAATCTGG | CAAGACTATGTGCCCTTGGA | 171 |