Figure 7
From: Hypoxic signature of microRNAs in glioblastoma: insights from small RNA deep sequencing
MiR-210-3p targets HIF3A. Effect of transient over-expression of miR-210-3p on HIF3A transcript levels was determined by qRT-PCR (a). Diagram showing wild type/mutated miR-210-3p binding site in the HIF3A 3′ UTR (b). 3′ UTR luciferase results obtained by cotransfection of luciferase constructs bearing wild type/mutated miR-210-3p binding sites in the HIF3A 3′ UTR, along with miR-210-3p over-expression vector (pBABE-miR-210) or the empty pBABE-puro parent vector (c). The graphical data points in a and c represent mean ± S.D. of at least three independent experiments. (*P > 0.01 and < 0.05; **P < 0.01). Error bars denote ± S. D.