Figure 8

Quantitative real-time rtPCR (qPCR) verification of biomarker gene response. A comparison of biomarker gene expressions measured by microarray (N = 3) and qPCR (N = 6) after 48 h waterborne exposure to 0.25, 0.5 and 1.0 mg/L nominal concentrations of depleted uranium (DU) in the liver of Atlantic salmon (Salmo salar). The lower and upper edge of box represent 25% median and 75% median of data, respectively, the middle line in the box represents the data median, the whiskers represent the data range (from the min. to max.). Left column: results from qPCR analysis; Right column: results from microarray analysis. a: not significantly different from the control; b: significantly different from the control; c: significantly different from 0.25 mg U/L treatment; d: significantly different from 0.5 mg U/L treatment.