Identification and grouping of rhoptry kinases. Rhoptry kinases (RopKs) were searched in the proteome of E. falciformis using HMMer3 and hidden markov models (HMMs) for specific RopK families developed by Talevich and Kannan . The figure shows the bitscore of the search against the general protein kinase HMM plotted against the score against best HMM in the database. Candidate rhoptry kinase proteins fall below the diagonal line where general kinases would be found and are labeled with the name of the respective best database HMM. The shape of symbols indicates the presence of a known T. gondii rhoptry kinase in the ortholog cluster of the protein, the color highlights the presence or absence of a signal peptide for secretion.