The genome of Eimeria falciformis - reduction and specialization in a single host apicomplexan parasite

BMC Genomics

Table 3 Eimeria falcifomis Rhoptry kinases (RopKs)

gene	Best HMM	Best score	General PK score	*E. tenella*ortholog	*T. gondii*ortholog
EfaB_MINUS_17096.g1521	ROPK-Eten3	252.90	95.60	ETH_00005415, ETH_00028835
EfaB_MINUS_32658.g2475	ROPK-Eten5	167.00	48.30	ETH_00002510
EfaB_MINUS_42996.g2710	ROP35	323.90	80.10	ETH_00005905	TGME49_104740
EfaB_MINUS_720.g57	ROPK-Unique	169.60	79.70	ETH_00005190	ROP18⁺⁺
EfaB_PLUS_15899.g1411	ROPK	146.20	86.60
EfaB_PLUS_24117.g1969	ROPK-Unique	188.30	3.00	ETH_00013325*
EfaB_PLUS_33184.g2393	ROPK-Unique	138.40	47.10	ETH_00005170
EfaB_PLUS_47595.g2679	ROP21-27	367.60	90.40		TGME49_113330
EfaB_PLUS_7742.g778	ROP21-27	391.80	86.80	ETH_00014495	TGME49_063220
EfaB_PLUS_8664.g829	ROP35	282.70	73.80	ETH_00026495

For putative E. falcifomis RopKs the family categorization obtained searching the HMMs developed by Talevich et al. [49] is given (best HMM). The score against this HMM can be compared with the score against the general protein-kinase-like profile (PKL score) to distinguish RopKs from universal kinases (see also Figure 4). Finally orthologs from E. tenella and T. gondii are given. The E. tenella ortholog marked with * is not included in the RopKs described by Talevich et al. [48], therefore EfaB_PLUS_24117.g1969 is not counted as having a RopK ortholog. Rop18 (marked with ⁺⁺) is not found in ortholog clusters. The E. falciformis gene, however, shows sequence similarity to Rop18-III from T. gondii (GI: 117574634).

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