Figure 2

sRNAs from F. cylindrus and E. huxleyi. Northern blot validation of F. cylindrus (a) and E. huxleyi (b) candidates and their expression in T. pseudonana (Tp), P. tricornutum (Pt), F. cylindrus (Fc), and E. huxleyi (Eh). ZR small RNA ladder (left) and EtBr gel (bottom) are provided for band sizing and to show equal loading of the above RNAs. (c) and (d), examples of F. cylindrus validated tRNAs producing sRNA sequences.The top visualisation represents the mapping pattern of sRNAs stacked up against the y-axis mapped to the tRNA sequence on the x-axis. The secondary structure of the tRNA is shown in dot-bracket notation above the x-axis. The middle graph shows the size class distribution of all sRNAs that map to the tRNA and the bottom schematic shows the folded tRNA with its most abundant sRNA from the library highlighted in red.