Table 2 Functional genomic annotation of phage UFV-P2
Gene | Predicted protein | Genomic coordinates | Strand | Protein mass (Da) | Protein pI | AA residues | Homologs (a) & Motifs | E-value |
|---|---|---|---|---|---|---|---|---|
ORF01 | Conserved hypothetical protein | 1205..1483 | + | 10486 | 10.5 | 92 | NP_955002 hypothetical protein PaP3p71 [Pseudomonas phage PaP3] | 7.34E-09 |
ORF02 | Conserved hypothetical protein | 1501..1983 | + | 17752 | 9.4 | 160 | YP_006382463 hypothetical protein tf_02 [Pseudomonas phage tf]; protein motifs: cl10333 PHA01782 | 3.22E-37 |
ORF03 | Conserved hypothetical protein | 2046..2216 | + | 6515 | 7.8 | 56 | YP_007183264 hypothetical protein BN425_ORF_56 [Pseudomonas phage vB_PaeP_p2-10_Or1] | 7.83E-02 |
ORF04 | Hypothetical protein | 2231..2368 | + | 5276 | 9.8 | 45 | - | - |
ORF05 | Hypothetical protein | 2365..2556 | + | 7171 | 9.2 | 63 | - | - |
ORF06 | Hypothetical membrane protein | 2553..2687 | + | 4708 | 9.5 | 44 | protein motifs: one transmembrane domain discovered using TMHMM and Phobius | |
ORF07 | Conserved hypothetical protein | 2677..2898 | + | 8374 | 9.8 | 73 | YP_006382470 hypothetical protein tf_10 [Pseudomonas phage tf] | 2.09E-04 |
ORF08 | Conserved hypothetical protein | 2898..3020 | + | 5178 | 9.3 | 40 | AGC35239 hypothetical protein PaP4_008 [Pseudomonas phage PaP4] | 1.19E-05 |
ORF09 | Hypothetical protein | 3021..3275 | + | 9120 | 4.1 | 84 | - | - |
ORF10 | Hypothetical protein | 3368..3568 | + | 7493 | 9.6 | 66 | - | - |
ORF11 | Hypothetical protein | 3555..3869 | + | 11403 | 5.0 | 104 | - | - |
ORF12 | Hypothetical protein | 3869..4063 | + | 7139 | 9.0 | 64 | - | - |
ORF13 | Conserved hypothetical protein | 4060..4458 | + | 14906 | 9.4 | 132 | YP_006382473 hypothetical protein tf_13 [Pseudomonas phage tf] | 9.55e-15 |
ORF14 | Hypothetical membrane protein | 4539..4736 | + | 7618 | 9.1 | 65 | protein motifs: one to two transmembrane domains discovered using TMHMM and Phobius | |
ORF15 | Conserved hypothetical protein | 4884..5714 | + | 29824 | 8.5 | 276 | AGC35249 transposase fusion protein [Pseudomonas phage PaP4]; protein motifs: pfam01145 Band_7, & cl02525 Band_7 | 1.15E-113 |
ORF16 | Conserved hypothetical protein | 5727..6017 | + | 10708 | 9.1 | 96 | YP_006659979 hypothetical protein tf_14 [Pseudomonas phage tf] | 2.29E-11 |
ORF17 | Conserved hypothetical protein | 6021..6149 | + | 4783 | 11.0 | 42 | NP_775206 hypothetical protein PaP3p51 [Pseudomonas phage PaP3] | 1.32E-03 |
ORF18 | Hypothetical protein | 6159..6374 | + | 7803 | 5.0 | 71 | - | - |
ORF19 | Conserved hypothetical protein | 6441..7289 | + | 31291 | 9.2 | 282 | YP_006382477 hypothetical protein tf_17 [Pseudomonas phage tf] | 1.75E-03 |
ORF20 | Conserved hypothetical protein | 7296..8159 | + | 32906 | 4.9 | 287 | YP_006382478 hypothetical protein tf_18 [Pseudomonas phage tf] | 2.06E-07 |
ORF21 | Conserved hypothetical protein | 8171..8968 | + | 29423 | 5.0 | 265 | YP_006382479 hypothetical protein tf_19 [Pseudomonas phage tf]; protein motifs: PF14395.1 COOH-NH2_lig | 8.75E-112 |
ORF22 | Conserved hypothetical protein | 8965..9525 | + | 21203 | 7.5 | 186 | NP_775210 hypothetical protein PaP3p47 [Pseudomonas phage PaP3] | 2.53E-40 |
ORF23 | Amidoligase | 9501..10658 | + | 43407 | 5.5 | 385 | YP_001671891 hypothetical protein [Pseudomonas phage LUZ24]; protein motifs: PF12224.3 Amidoligase_2 | 4.46E-96 |
ORF24 | HNH endonuclease | 10667..11071 | + | 15340 | 9.6 | 134 | YP_002003475 gp2.8 [Enterobacteria phage BA14]; protein motifs: PF13392.1 HNH_3 | 2.71E-20 |
ORF25 | Glutamine amidotransferase | 11084..12544 | + | 54036 | 5.9 | 486 | YP_006382482 glutamine amidotransferase [Pseudomonas phage tf]; protein motifs: PF13522.1 GATase_6, & cd00352 Gn_AT_II | 2.72E-151 |
ORF26 | Conserved hypothetical protein | 12547..12765 | + | 8166 | 4.8 | 72 | NP_775213 hypothetical protein PaP3p44 [Pseudomonas phage PaP3] | 2.25E-20 |
ORF27 | Hypothetical protein | 12805..12909 | + | 3505 | 5.3 | 34 | - | - |
ORF28 | ATP-grasp enzyme | 12906..13781 | + | 31797 | 7.7 | 291 | YP_006382486 hypothetical protein tf_24 [Pseudomonas phage tf] | 2.60E-96 |
ORF29 | Conserved hypothetical protein | 13774..14169 | + | 15076 | 5.5 | 131 | AGC35259 hypothetical protein PaP4_028 [Pseudomonas phage PaP4]; proteins motifs: PF06094.7 AIG2, & cd06661 GGCT_like | 5.55E-27 |
ORF30 | Conserved hypothetical protein | 14169..14465 | + | 11295 | 5.4 | 98 | YP_006382488 hypothetical protein tf_026 [Pseudomonas phage tf] | 6.03E-23 |
ORF31 | DNA primase/helicase | 14425..16176 | + | 65673 | 5.9 | 583 | YP_001671897 primase/helicase [Pseudomonas phage LUZ24]; protein motifs: PF13155.1 Toprim_2, & PF03796.10 DnaB_C | 0 |
ORF32 | DNA polymerase part I | 16151..16666 | + | 20133 | 4.5 | 171 | YP_006382490 3′-5′ exonuclease [Pseudomonas phage tf] & YP_001671898 DNA polymerase part I [Pseudomonas phage LUZ24] | 2.82E-75; 3.72e-68 |
ORF33 | Conserved hypothetical membrane protein | 16669..16920 | + | 9085 | 10.0 | 83 | YP_006382491 hypothetical protein tf_29 [Pseudomonas phage tf]; protein motifs: one transmembrane domain discovered using TMHMM and Phobius | 1.99E-06 |
ORF34 | Hypothetical protein | 16931..17068 | + | 5096 | 3.4 | 45 | - | - |
ORF35 | Hypothetical protein | 17095..17325 | + | 8505 | 9.0 | 76 | - | - |
ORF36 | Conserved hypothetical protein | 17336..17539 | + | 7596 | 5.8 | 67 | YP_001671900 hypothetical protein [Pseudomonas phage LUZ24] | 2.23E-02 |
ORF37 | Putative holin | 17536..17817 | + | 10475 | 9.8 | 93 | YP_006382493 hypothetical protein tf_32 [Pseudomonas phage tf] & YP_001671904 putative holin [Pseudomonas phage LUZ24]; protein motifs: three transmembrane domains discovered using TMHMM and Phobius | 5.03e-35; 1.92e-18 |
ORF38 | Hypothetical membrane protein | 17814..18032 | + | 8079 | 9.9 | 72 | protein motifs: one or two transmembrane domains discovered using TMHMM and Phobius | |
ORF39 | Conserved hypothetical protein | 18069..18263 | + | 6728 | 3.5 | 64 | YP_006382494 hypothetical protein tf_34 [Pseudomonas phage tf] | 6.48E-04 |
ORF40 | Conserved hypothetical protein | 18263..18433 | + | 5628 | 8.2 | 56 | YP_007112538 hypothetical protein MAR_61 [Vibrio phage vB_VpaM_MAR] | 9.20E-05 |
ORF41 | Conserved hypothetical protein | 18433..18627 | + | 7272 | 10.9 | 64 | YP_006382495 hypothetical protein tf_35 [Pseudomonas phage tf] | 1.52E-03 |
ORF42 | Hypothetical protein | 18630..18764 | + | 4887 | 6.0 | 44 | - | - |
ORF43 | DNA polymerase part II | 18765..20405 | + | 60865 | 9.6 | 546 | YP_007183240 DNA polymerase1 [Pseudomonas phage vB_PaeP_p2-10_Or1] & YP_001671907 DNA polymerase part II [Pseudomonas phage LUZ24]; protein motifs: PF00476.15 DNA_pol_A | 0; 0 |
ORF44 | DNA binding protein | 20472..21068 | + | 21853 | 4.8 | 198 | YP_006382500 DNA binding protein [Pseudomonas phage tf] | 6.61E-70 |
ORF45 | Conserved hypothetical protein | 21139..21495 | + | 13200 | 6.3 | 118 | AGC35272 hypothetical protein PaP4_041 [Pseudomonas phage PaP4] | 4.02E-47 |
ORF46 | Hypothetical protein | 21525..21755 | + | 8335 | 4.7 | 76 | - | - |
ORF47 | Hypothetical protein | 21887..22222 | + | 12649 | 9.4 | 111 | - | - |
ORF48 | 5′-3′ exonuclease | 22219..23103 | + | 33498 | 5.3 | 294 | NP_775229 exonuclease [Pseudomonas phage PaP3]; protein motifs: PF01367.15 5_3_exonuc, & cd09898 H3TH_53EXO | 1.47E-149 |
ORF49 | Conserved hypothetical protein | 23078..24073 | + | 37154 | 4.7 | 331 | YP_006659984 conserved hypothetical protein [Pseudomonas phage tf] | 1.15E-39 |
ORF50 | Endonuclease | 23874..24341 | + | 17655 | 5.4 | 155 | YP_006382505 endonuclease [Pseudomonas phage tf] | 1.11E-41 |
ORF51 | Conserved hypothetical protein | 24307..25062 | + | 28981 | 6.2 | 251 | YP_001671917 hypothetical protein [Pseudomonas phage LUZ24] | 2.15E-129 |
ORF52 | Hypothetical protein | 25059..25184 | + | 4844 | 10.5 | 41 | - | - |
ORF53 | Conserved hypothetical protein | 25255..25458 | + | 7717 | 6.5 | 67 | YP_006659986 hypothetical protein tf_48 [Pseudomonas phage tf] | 7.14E-14 |
ORF54 | Hypothetical protein | 25455..25631 | + | 6814 | 4.6 | 58 | - | - |
ORF55 | Conserved hypothetical protein | 25628..25834 | + | 8109 | 4.4 | 68 | YP_001671920 hypothetical protein [Pseudomonas phage LUZ24] | 1.57E-12 |
ORF56 | Conserved hypothetical protein | 25963..26319 | - | 13124 | 5.9 | 118 | AGC35282 hypothetical protein PaP4_051 [Pseudomonas phage PaP4] | 2.11E-45 |
ORF57 | Phage structural protein | 26321..27208 | - | 32029 | 5.5 | 295 | AFD10698 hypothetical protein I7C_020 [Pseudomonas phage MR299-2] | 3.43E-152 |
ORF58 | Phage structural protein | 27219..30383 | - | 111420 | 5.3 | 1054 | YP_001671923 phage particle protein [Pseudomonas phage LUZ24] | 0 |
ORF59 | Phage structural protein | 30389..32101 | - | 60235 | 5.5 | 570 | YP_001671924 phage particle protein [Pseudomonas phage LUZ24] | 1.35E-86 |
ORF60 | Phage structural protein | 32103..32507 | - | 13485 | 6.5 | 134 | YP_001671925 phage particle protein [Pseudomonas phage LUZ24] | 9.26E-11 |
ORF61 | Phage particle protein | 32504..33448 | - | 32240 | 4.8 | 314 | AFD10694 hypothetical protein I7C_016 [Pseudomonas phage MR299-2] | 3.23E-112 |
ORF62 | Conserved hypothetical protein | 33429..33863 | - | 16522 | 4.5 | 144 | AGC35288 hypothetical protein PaP4_057 [Pseudomonas phage PaP4] | 2.95E-57 |
ORF63 | Conserved hypothetical protein | 33860..34570 | - | 25585 | 4.8 | 236 | YP_001671928 hypothetical protein [Pseudomonas phage LUZ24] | 3.73E-56 |
ORF64 | Phage particle protein | 34570..36111 | - | 57542 | 5.1 | 513 | YP_006382515 phage particle protein [Pseudomonas phage tf] & YP_001671929 hypothetical protein [Pseudomonas phage LUZ24] | 0; 0 |
ORF65 | Tail fiber protein | 36119..36754 | - | 21879 | 6.7 | 211 | NP_775246 hypothetical protein PaP3p12 [Pseudomonas phage PaP3] | 1.68E-62 |
ORF66 | Conserved hypothetical protein | 36976..37170 | - | 6838 | 6.6 | 64 | YP_001671932 hypothetical protein LUZ24 [Pseudomonas phage LUZ24] | 3.02E-28 |
ORF67 | phage structural protein | 37175..37804 | - | 23887 | 5.0 | 209 | AFD10687 putative constituent protein [Pseudomonas phage MR299-2] | 2.54E-82 |
ORF68 | Conserved hypothetical protein | 37808..38128 | - | 11847 | 5.8 | 106 | YP_007183215 hypothetical protein BN425_ORF_07 [Pseudomonas phage vB_PaeP_p2-1 0_Or1] | 1.26E-50 |
ORF69 | Major capsid protein | 38180..39133 | - | 34740 | 6.0 | 317 | NP_775251 major head protein [Pseudomonas phage PaP3] & YP_001671935 major head protein [Pseudomonas phage LUZ24] | 0; 0 |
ORF70 | Scaffolding protein | 39152..40138 | - | 36190 | 4.3 | 328 | YP_001671936 scaffolding protein [Pseudomonas phage LUZ24] | 4.90E-101 |
ORF71 | Conserved hypothetical protein | 40128..40379 | - | 9559 | 5.2 | 83 | AGC35298 hypothetical protein PaP4_067 [Pseudomonas phage PaP4] & YP_001671937 hypothetical protein [Pseudomonas phage LUZ24] | 8.9E-30; 2.00e-29 |
ORF72 | Portal protein | 40379..42469 | - | 79405 | 5.0 | 696 | YP_007183212 putative portal protein [Pseudomonas phage vB_PaeP_p2-10_Or1] YP_001671938 portal protein [Pseudomonas phage LUZ24] | 0; 0 |
ORF73 | Terminase, large subunit | 42496..43941 | - | 54330 | 6.0 | 481 | YP_001671939 terminase large subunit [Pseudomonas phage LUZ24]; protein motifs: PF03237.10 Terminase_6 | 0 |
ORF74 | Lysozyme | 43945..44451 | - | 18961 | 8.6 | 168 | YP_006382529 lysozyme [Pseudomonas phage tf]; protein motifs: PF00959.14 Phage_lysozyme | 3.27E-56 |
ORF75 | Terminase, small subunit | 44372..44845 | - | 17291 | 5.9 | 157 | YP_007183209 hypothetical protein BN425_ORF_01 [Pseudomonas phage vB_PaeP_p2-1 0_Or1] & YP_006382530 terminase small subunit [Pseudomonas phage tf] | 5.76E-66; 2.59e-64 |