Effect of LNA-122i treatment on hepatic abundance and phosphorylation status of key elements of the hepatic insulin signaling pathway. Phosphorylated and total protein abundance was measured for AKT (A), mTOR (B), S6 (C), AMPK (D), FoxO1A (E), GSK3β (F). The individual graphs show the relative abundance of the phosphorylated form, the total protein and the ratio between the relative abundance of the phosphorylated form and the relative abundance of the total protein. All protein abundances were normalized by β-tubulin abundance, which did not change significantly with treatment (df = 2; F = 0.949; p > 0.05, data not shown).Representative Western Blot images are shown (G). Analyzed groups consisted of n = 6 samples and data are depicted as mean ± S.E.M. for each group. Data were analyzed using one-way ANOVA and differences between individual experimental groups assessed by Student-Newman-Keuls post-hoc test. Different letters indicate a significant difference (p < 0.05) between experimental groups.