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Table 2 Overrepresented GO terms in leaves and roots DEGs

From: Identification of candidate genes involved in early iron deficiency chlorosis signaling in soybean (Glycine max) roots and leaves

Leaves description: biological processes

GO terms

DEGs

P-value

DNA unwinding involved in replication

GO:0006268

7

0

Membrane disassembly

GO:0030397

12

0

Wax biosynthesis and metabolism

GO:0010025, GO:0010166

12

0

Defense response to bacterium

GO:0009816

14

0

DNA methylation

GO:0006306

11

0

Lipid metabolism

GO:0006629

76

0

Single-organism biosynthesis and metabolism

GO:0044710, GO:0044711

167

0

Organic hydroxy compound biosynthesis and metabolism

GO:0006066, GO:1901615, GO:1901617

30

0.005

Negative regulation of developmental growth

GO:0048640

3

0.007

Response to stimulus

GO:0050896

197

0.007

Cuticle development

GO:0042335

9

0.009

Root development and morphogenesis

GO:0010101, GO:0010015, GO:0010311, GO:0022411, GO:0048528

30

0.01

Cellular response to sucrose starvation, mannitol and sorbitol

GO:0018008, GO:0018201, GO:0043617, GO:0071325, GO:0072709

3

0.012

Organic acid biosynthesis

GO:0016053, GO:0046394, GO:0072330

50

0.041

Leaves description: molecular functions

  

Xyloglucan:xyloglucosyl transferase activity

GO:0016762

11

0

Transferase activity, transferring acyl groups

GO:0016746

25

0

Tetrapyrrole binding

GO:0046906

20

0.004

Oxidoreductase activity

GO:0016491, GO:0016661, GO:0016701, GO:0016702

59

0.029

Phosphatidylinositol binding

GO:0005547, GO:0043325

2

0.033

Carboxylic ester hydrolase activity

GO:0052689

18

0.033

Catalytic activity

GO:0003824

209

0.041

Roots description: biological processes

GO Terms

DEGs

P -value

Cell junction organization and assembly

GO:0034329, GO:0034330

12

0

Root development

GO:0009913, GO:0010053, GO:0022622, GO:0048364, GO:0048640

40

0

Response to ethylene and other stimuli

GO:0050896, GO:0070887, GO:0071369

198

0

Phenylpropanoid biosynthesis and metabolism

GO:0009698, GO:0009699, GO:0009812, GO:0009813, GO:0009962, GO:0009963, GO:0043455, GO:1900376, GO:1900378, GO:2000762

27

0.002

Response to oxidative and other stress

GO:0006950, GO:0006979

155

0.002

Zinc ion transmembrane transport

GO:0006829, GO:0071577

6

0.003

Steroid biosynthesis and metabolism

GO:0006694, GO:0008202

16

0.009

Coumarin biosynthesis and metabolism

GO:0009804, GO:0009805

9

0.015

N-terminal peptidyl-glycine N-myristoylation

GO:0018008

2

0.021

Immune system process

GO:0002376

45

0.027

Cellular response to starvation

GO:0009267

23

0.03

Single-organism biosynthesis and metabolism

GO:0044710, GO:0044711

140

0.036

Cellular amine metabolic process

GO:0044106

12

0.038

Cell wall organization or biogenesis

GO:0071554

44

0.039

Cell communication

GO:0007154

85

0.044

Roots description: molecular functions

  

Antioxidant activity

GO:0016209, GO:0016684

22

0

Tetrapyrrole binding

GO:0046906

26

0

Oxidoreductase activity

GO:0016491

58

0.002

Protein homodimerization activity

GO:0042803

13

0.002

Zinc ion transmembrane transporter activity

GO:0005385

6

0.002

Regulatory region nucleic acid binding

GO:0001067

32

0.003

ADP binding

GO:0043531

17

0.005

Identical protein binding

GO:0042802

16

0.005

  1. To determine gene ontology terms overrepresented among differentially expressed genes in leaves or roots, Ontologizer 2.0 software [50] was used with parent–child-union analysis and Westfall-Young-Single-Step multiple testing correction, with a resampling of 1000 replicates. GO terms were combined when Glyma IDs overlapped entirely between two or more terms. The term containing the largest number of genes is in bold, with its corresponding P-value reported.