Figure 3
From: Using Galaxy-P to leverage RNA-Seq for the discovery of novel protein variations
Comparison of score distributions of all peptides identified in the search versus peptides containing SAPs. For Jurkat cells, the distribution of SEQUEST XCorr Scores for peptides passing a 1% false discovery rate were compared between 1) peptides mapping to the Ensembl reference proteome, and 2) peptides containing single amino acid polymorphisms (SAPs) derived from the sample-matched RNA-Seq data. SAP-containing peptides had, on average, higher peptide spectral match (PSM) quality scores as compared to those of reference peptides, attesting to the high quality of the sample-specific SAP database employed for MS searching.