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Figure 3 | BMC Genomics

Figure 3

From: Using Galaxy-P to leverage RNA-Seq for the discovery of novel protein variations

Figure 3

Comparison of score distributions of all peptides identified in the search versus peptides containing SAPs. For Jurkat cells, the distribution of SEQUEST XCorr Scores for peptides passing a 1% false discovery rate were compared between 1) peptides mapping to the Ensembl reference proteome, and 2) peptides containing single amino acid polymorphisms (SAPs) derived from the sample-matched RNA-Seq data. SAP-containing peptides had, on average, higher peptide spectral match (PSM) quality scores as compared to those of reference peptides, attesting to the high quality of the sample-specific SAP database employed for MS searching.

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