qPCR analysis of miR-200 family and in situ hybridization of miR200a. Expression of all three members of the chicken miR-200 family is significantly upregulated in ovarian tumors compared to normal ovaries. Expression of all three members is significantly decreased in ovarian tumors from flax-fed hens compared to tumors from control-fed hens. Expression of miR-200a is confined to the tumor cells of the control-fed ovary (A) and flax-fed ovary (B). Little if any expression is observed in normal ovary tissue (C). Magnification 20x on left, 100x on right.