ZEB1 mRNA and immunohistochemistry. qPCR of ZEB1 mRNA indicates an upregulation in tumors from both control and flax fed hens but no nuclear expression by immunohistochemistry in the tumor epithelium (control-fed top, flax-fed bottom). Some nuclear expression is seen in adjacent normal stroma from flax fed hen (bottom, arrows). ZEB1 is red, E-cadherin is green, nuclei are stained with DAPI. Smaller micrographs include the blue DAPI channel. Magnification at 100x.