Sequence mapping and verification of 5 genes with exon skipping events detected by RNA-seq by PCR and RT-PCR. The expression profiles of the same gene in female (red) and male (blue) parasites were placed under the line representing the chromosome position. The black lines represent original annotated gene structures (thick lines indicate exonic regions, and thin lines indicate intronic regions), while the active transcripts in red and blue identified from the same genes in female and male parasites are underneath. The five genes and transcripts (A, B, C, D, E) with exon skipping events were confirmed by PCR and RT-PCR (F). gE indicates PCR products amplified from genomic DNA, and cE indicates PCR products amplified from cDNA. Red arrows indicate transcripts generated by exon skipping, and green arrows indicate primer locations.