Figure 4

Sequence mapping and verification of three genes with intron retention events detected by RNA-seq by PCR and RT-PCR. The three genes and transcripts (A, B, C) with intron retention events were confirmed by PCR and RT-PCR (D). gI indicates PCR products amplified from genomic DNA, and cI indicates PCR products amplified from cDNA. Red arrows indicate transcripts generated by intron retention, and green arrows indicate primer locations.