Table 5 Summary of experimental design for RNA-seq experiments
Experiment | Biological replicates | Individuals pooled per replicate | Tissues sequenced | Total number of libraries | HiSeq2000 lanes | Read types | Average number of reads per library |
|---|---|---|---|---|---|---|---|
Sex-specific antennal expression | 1 | 25 females or 11 males | antennae (two per individual) | 2 | 0.7 | 100 bp paired-end | 81.5×106 |
Tissue-specific expression in females | 3 | 20 females | antennae (two per individual), heads, legs (six per individual), thoraces & abdomens | 12 | 1 | 100 bp single-end | 18×106 |