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Table 2 Results of CCS and cluster analyses of the V1R I and V1R IX repertoires of two individuals of M. murinus

From: The utility of PacBio circular consensus sequencing for characterizing complex gene families in non-model organisms

 

M. murinus1

M. murinus2

Raw CCS reads

29,739

32,837

Post quality filter

16,914

17,174

V1RI CCS reads

12,625

11,814

V1RIX CCS reads

4,289

5,360

V1RI Clusters (≥7×)

106

114

V1RIX Clusters (≥7×)

61

85

CCS V1RI loci (98 to 99%)

22–28

20–27

CCS V1RIX loci (98 to 99%)

20–24

20–28

Estimated V1RI repertoire

34 (30)1

36

Estimated V1RIX repertoire

32 (19)1

28

  1. PacBio CCS V1R subfamily loci are estimates based on 98% to 99% genetic similarity thresholds (see Methods). Final repertoire estimates are based on cluster analyses of all available V1RI and V1RIX data (e.g. Young et al.[18], Yoder et al.[29], and this study). Chimera detection results are presented in Additional file 1: Figure S2. 1Number of functional genes mined from the draft M. murinus genome by Young et al.[18].
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BMC Genomics

ISSN: 1471-2164

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