Figure 3

Classification of glomerular microvascular endothelial cell (HGMEC) library as endothelial. A) Cross-validation using 63 non-endothelial and 18 endothelial SAGE-libraries not including HGMEC SAGE library. The minimum number of false classifications (false positive: a non-endothelial library classified as endothelial and false negative: an endothelial library classified as non-endothelial) was observed for n = 100 or 150 tags. B) Histogram showing the sum of relative expression for each of the endothelial and non-endothelial SAGE libraries as used for cross-validation in panel A). The sum of relative tag counts of 150 tags discriminates between endothelial and non-endothelial SAGE libraries. If the sum is larger than the threshold 0.022, the library is characterised as endothelial. This threshold is clearly exceeded by the HGMEC SAGE library (0.07).