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Table 2 Expression of the 50 most abundant from 268 tags which were common to HGMEC and ex vivo glomerular SAGE libraries and overexpressed in HGMEC in comparison to the cumulative endothelial cell library

From: A SAGE based approach to human glomerular endothelium: defining the transcriptome, finding a novel molecule and highlighting endothelial diversity

UniGene P-value FDR corrected p-value Endo HGMEC Expected HGMEC Description Confirmed by RT-PCR
hs.425125 0.00E + 000 0.00E + 000 145 551 88.38 Ribosomal protein L29 (Hs00988959_gH) +
hs.514581 0.00E + 000 0.00E + 000 103 482 74.29 Actin, gamma 1  
hs.586423 0.00E + 000 0.00E + 000 29 434 58.80 Eukaryotic translation elongation factor 1 alpha 1 (Hs00265885_g1) +
hs.182825 0.00E + 000 0.00E + 000 83 413 62.99 Ribosomal protein L35  
hs.632703 0.00E + 000 0.00E + 000 8 407 52.70 Ribosomal protein L41  
hs.445351 0.00E + 000 0.00E + 000 33 394 54.22 Lectin, galactoside-binding, soluble, 1 (galectin 1)  
hs.522584 0.00E + 000 0.00E + 000 18 380 50.54 Thymosin beta 4, X-linked (Hs03406519_gH) +
hs.374596 0.00E + 000 0.00E + 000 53 353 51.56 Tumor protein, translationally-controlled 1  
hs.91481 0.00E + 000 0.00E + 000 24 319 43.56 EGF-like-domain, multiple 7 (Hs00211952_m1) +
hs.523185 0.00E + 000 0.00E + 000 62 286 44.19 Ribosomal protein L13a  
hs.524910 0.00E + 000 0.00E + 000 32 252 36.06 Ferritin, heavy polypeptide 1  
hs.546286 0.00E + 000 0.00E + 000 23 237 33.02 Ribosomal protein S3 (Hs02385124_g1) +
hs.265174 1.96E-305 1.24E-303 9 215 28.45 Ribosomal protein L32  
hs.494691 1.20E-288 7.05E-287 199 355 70.35 Profilin 1 (Hs00277097_m1) +
hs.446574 4.37E-285 2.40E-283 13 206 27.81 Thymosin beta 10 (Hs00363670_m1) +
hs.400295 4.05E-268 2.08E-266 0 179 22.73 Ribosomal protein L30  
hs.388664 8.04E-253 3.89E-251 49 220 34.16 Ribosomal protein L11  
hs.546269 4.36E-246 1.99E-244 130 277 51.68 Ribosomal protein L10a  
hs.433427 1.91E-226 8.27E-225 45 198 30.86 Ribosomal protein S17  
hs.515070 1.04E-224 4.28E-223 28 181 26.54 Eukaryotic translation elongation factor 2  
hs.144835 4.47E-213 1.75E-211 66 206 34.54 Eukaryotic translation elongation factor 1 gamma  
hs.523463 9.00E-212 3.37E-210 50 192 30.73 Ribosomal protein L27a (Hs00741143_s1) +
hs.275243 9.12E-204 3.26E-202 0 136 17.27 S100 calcium binding protein A6 (Hs01002197_g1) +
hs.654404 1.62E-196 5.56E-195 182 269 57.27 Major histocompatibility complex, class I, C  
hs.437594 1.55E-187 5.10E-186 79 197 35.05 Ribosomal protein, large, P2  
hs.397609 1.12E-182 3.55E-181 12 136 18.79 Ribosomal protein S16  
hs.111779 1.18E-178 3.60E-177 32 153 23.49 Secreted protein, acidic, cysteine-rich (osteonectin) (Hs00234160_m1) +
hs.438429 1.19E-170 3.50E-169 177 244 53.46 Ribosomal protein S19  
hs.605502 4.11E-150 1.17E-148 12 114 16.0 Heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa) (Hs00607129_gH) +
hs.546288 8.08E-149 2.22E-147 144 207 44.57 Ribosomal protein S9  
hs.632717 1.24E-145 3.29E-144 88 171 32.89 Myosin, light chain 6, alkali, smooth muscle and non-muscle (Hs02597812_g1) +
hs.627414 3.86E-142 9.93E-141 102 177 35.43 Ribosomal protein S18  
hs.381219 1.80E-138 4.49E-137 10 104 14.48 Ribosomal protein L15  
hs.410817 7.03E-122 1.70E-120 200 211 52.19 Ribosomal protein L13  
hs.511605 3.74E-121 8.79E-120 21 103 15.75 Annexin A2 (Hs00743063_s1) +
hs.17441 6.13E-121 1.40E-119 28 109 17.40 Collagen, type IV, alpha 1 (Hs00266237_m1) +
hs.520898 9.10E-117 2.02E-115 6 85 11.56 Cathepsin B (Hs00947433_m1) +
hs.356572 2,11E-115 2,11E-115 63 132 24,76 Ribosomal protein S3a  
hs.170622 1.25E-112 2.64E-111 378 275 82.92 Cofilin 1 (non-muscle) (Hs02621564_g1) +
hs.278573 2.63E-112 5.41E-111 6 82 11.17 CD59 molecule, complement regulatory protein (Hs00174141_m1) +
hs.433701 1.37E-109 2.75E-108 126 163 36.70 Ribosomal protein L37a  
hs.8372 2.40E-104 4.70E-103 23 93 14.73 Ubiquinol-cytochrome c reductase, 6.4 kDa subunit  
hs.527193 9.87E-103 1.89E-101 22 91 14.35 Ribosomal protein S23  
hs.387208 8.69E-096 1.63E-094 13 78 11.56 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed  
hs.437191 1.83E-094 3.35E-093 23 86 13.84 Polymerase I and transcript release factor (Hs00396859_m1) +
hs.591346 6.61E-094 1.18E-092 63 114 22.48 Connective tissue growth factor (Hs01026926_g1) +
hs.644628 1.40E-091 2.45E-090 167 166 42.29 Ribosomal protein L4  
hs.77961 2.67E-089 4.58E-088 44 98 18.03 Major histocompatibility complex, class I, B  
hs.594444 2.09E-088 3.51E-087 8 68 9.65 Lamin A/C (Hs00153462_m1) +
hs.446628 4.24E-087 6.98E-086 81 119 25.40 Ribosomal protein S4, X-linked  
  1. UniGene numbers represent the UniGene groups to which the tags are assigned. P-value refers to expression comparison between HGMEC and Endo. FDR: multiple test correction using false discovery rate. Endo: Detected total copy number in the non-glomerular endothelial reference SAGE library. HGMEC: Detected copy number in HGMEC. Expected HGMEC: number of this particular tag statistically expected in HGMEC SAGE library by means of Chi-square statistics; Confirmed by RT-PCR: for these randomly chosen tags (n = 20) qRT-PCR was performed to confirm their expression in HGMEC. +: confirmation by qRT-PCR was successful (thus expression of all 20 transcripts confirmed by a TaqMan® Assay; each assay ID is shown in brackets).