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Table 2 Expression of the 50 most abundant from 268 tags which were common to HGMEC and ex vivo glomerular SAGE libraries and overexpressed in HGMEC in comparison to the cumulative endothelial cell library

From: A SAGE based approach to human glomerular endothelium: defining the transcriptome, finding a novel molecule and highlighting endothelial diversity

UniGene

P-value

FDR corrected p-value

Endo

HGMEC

Expected HGMEC

Description

Confirmed by RT-PCR

hs.425125

0.00E + 000

0.00E + 000

145

551

88.38

Ribosomal protein L29 (Hs00988959_gH)

+

hs.514581

0.00E + 000

0.00E + 000

103

482

74.29

Actin, gamma 1

 

hs.586423

0.00E + 000

0.00E + 000

29

434

58.80

Eukaryotic translation elongation factor 1 alpha 1 (Hs00265885_g1)

+

hs.182825

0.00E + 000

0.00E + 000

83

413

62.99

Ribosomal protein L35

 

hs.632703

0.00E + 000

0.00E + 000

8

407

52.70

Ribosomal protein L41

 

hs.445351

0.00E + 000

0.00E + 000

33

394

54.22

Lectin, galactoside-binding, soluble, 1 (galectin 1)

 

hs.522584

0.00E + 000

0.00E + 000

18

380

50.54

Thymosin beta 4, X-linked (Hs03406519_gH)

+

hs.374596

0.00E + 000

0.00E + 000

53

353

51.56

Tumor protein, translationally-controlled 1

 

hs.91481

0.00E + 000

0.00E + 000

24

319

43.56

EGF-like-domain, multiple 7 (Hs00211952_m1)

+

hs.523185

0.00E + 000

0.00E + 000

62

286

44.19

Ribosomal protein L13a

 

hs.524910

0.00E + 000

0.00E + 000

32

252

36.06

Ferritin, heavy polypeptide 1

 

hs.546286

0.00E + 000

0.00E + 000

23

237

33.02

Ribosomal protein S3 (Hs02385124_g1)

+

hs.265174

1.96E-305

1.24E-303

9

215

28.45

Ribosomal protein L32

 

hs.494691

1.20E-288

7.05E-287

199

355

70.35

Profilin 1 (Hs00277097_m1)

+

hs.446574

4.37E-285

2.40E-283

13

206

27.81

Thymosin beta 10 (Hs00363670_m1)

+

hs.400295

4.05E-268

2.08E-266

0

179

22.73

Ribosomal protein L30

 

hs.388664

8.04E-253

3.89E-251

49

220

34.16

Ribosomal protein L11

 

hs.546269

4.36E-246

1.99E-244

130

277

51.68

Ribosomal protein L10a

 

hs.433427

1.91E-226

8.27E-225

45

198

30.86

Ribosomal protein S17

 

hs.515070

1.04E-224

4.28E-223

28

181

26.54

Eukaryotic translation elongation factor 2

 

hs.144835

4.47E-213

1.75E-211

66

206

34.54

Eukaryotic translation elongation factor 1 gamma

 

hs.523463

9.00E-212

3.37E-210

50

192

30.73

Ribosomal protein L27a (Hs00741143_s1)

+

hs.275243

9.12E-204

3.26E-202

0

136

17.27

S100 calcium binding protein A6 (Hs01002197_g1)

+

hs.654404

1.62E-196

5.56E-195

182

269

57.27

Major histocompatibility complex, class I, C

 

hs.437594

1.55E-187

5.10E-186

79

197

35.05

Ribosomal protein, large, P2

 

hs.397609

1.12E-182

3.55E-181

12

136

18.79

Ribosomal protein S16

 

hs.111779

1.18E-178

3.60E-177

32

153

23.49

Secreted protein, acidic, cysteine-rich (osteonectin) (Hs00234160_m1)

+

hs.438429

1.19E-170

3.50E-169

177

244

53.46

Ribosomal protein S19

 

hs.605502

4.11E-150

1.17E-148

12

114

16.0

Heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa) (Hs00607129_gH)

+

hs.546288

8.08E-149

2.22E-147

144

207

44.57

Ribosomal protein S9

 

hs.632717

1.24E-145

3.29E-144

88

171

32.89

Myosin, light chain 6, alkali, smooth muscle and non-muscle (Hs02597812_g1)

+

hs.627414

3.86E-142

9.93E-141

102

177

35.43

Ribosomal protein S18

 

hs.381219

1.80E-138

4.49E-137

10

104

14.48

Ribosomal protein L15

 

hs.410817

7.03E-122

1.70E-120

200

211

52.19

Ribosomal protein L13

 

hs.511605

3.74E-121

8.79E-120

21

103

15.75

Annexin A2 (Hs00743063_s1)

+

hs.17441

6.13E-121

1.40E-119

28

109

17.40

Collagen, type IV, alpha 1 (Hs00266237_m1)

+

hs.520898

9.10E-117

2.02E-115

6

85

11.56

Cathepsin B (Hs00947433_m1)

+

hs.356572

2,11E-115

2,11E-115

63

132

24,76

Ribosomal protein S3a

 

hs.170622

1.25E-112

2.64E-111

378

275

82.92

Cofilin 1 (non-muscle) (Hs02621564_g1)

+

hs.278573

2.63E-112

5.41E-111

6

82

11.17

CD59 molecule, complement regulatory protein (Hs00174141_m1)

+

hs.433701

1.37E-109

2.75E-108

126

163

36.70

Ribosomal protein L37a

 

hs.8372

2.40E-104

4.70E-103

23

93

14.73

Ubiquinol-cytochrome c reductase, 6.4 kDa subunit

 

hs.527193

9.87E-103

1.89E-101

22

91

14.35

Ribosomal protein S23

 

hs.387208

8.69E-096

1.63E-094

13

78

11.56

Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed

 

hs.437191

1.83E-094

3.35E-093

23

86

13.84

Polymerase I and transcript release factor (Hs00396859_m1)

+

hs.591346

6.61E-094

1.18E-092

63

114

22.48

Connective tissue growth factor (Hs01026926_g1)

+

hs.644628

1.40E-091

2.45E-090

167

166

42.29

Ribosomal protein L4

 

hs.77961

2.67E-089

4.58E-088

44

98

18.03

Major histocompatibility complex, class I, B

 

hs.594444

2.09E-088

3.51E-087

8

68

9.65

Lamin A/C (Hs00153462_m1)

+

hs.446628

4.24E-087

6.98E-086

81

119

25.40

Ribosomal protein S4, X-linked

 
  1. UniGene numbers represent the UniGene groups to which the tags are assigned. P-value refers to expression comparison between HGMEC and Endo. FDR: multiple test correction using false discovery rate. Endo: Detected total copy number in the non-glomerular endothelial reference SAGE library. HGMEC: Detected copy number in HGMEC. Expected HGMEC: number of this particular tag statistically expected in HGMEC SAGE library by means of Chi-square statistics; Confirmed by RT-PCR: for these randomly chosen tags (n = 20) qRT-PCR was performed to confirm their expression in HGMEC. +: confirmation by qRT-PCR was successful (thus expression of all 20 transcripts confirmed by a TaqMan® Assay; each assay ID is shown in brackets).
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BMC Genomics

ISSN: 1471-2164

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