Figure 3

Gene expression patterns in purified BAL macrophages. (a) PCA plot as in Figure 1a for purified macrophages from WT (Squares) and Scnn1b-Tg (Triangles) mice at PNDs 0 (red), 3 (green), 10 (blue), 42 (purple) and for germ-free (GF) macrophages at PND 42 (orange). PC#1 (22% of the overall variance) separates the PND 0 from other ages; PC#2 (6.6% of the overall variance) separates WT from Scnn1b-Tg. (b) Unsupervised hierarchical clustering of the combined set of developmentally regulated DEGs as determined for Figure 1b except from purified macrophages (total number of genes represented = 4763; Additional file 2: Results file S1). (c) Unsupervised hierarchical clustering of the combined set of DEGs in macrophages filtered as in Figure 2 for WT versus Scnn1b-Tg (total genes represented = 1320; Additional file 2: Results file S1). For this figure, symbols, color coding and filtering were as in Figure 1. GF = germ-free.