Figure 5

A subset of human ORs is expressed in SN and down-regulated in PD post mortem brains. a) FANTOM5 CAGE data in human SN samples. Heat map graphical representation of human ORs expression in adult and neonate samples, as indicated. List of human ORs identified by genomic approach (left) and by homology search (right). b) Complete open reading frame for human OR2L13 was cloned from RNA extracted from SN of two individuals (SN#1 and SN#2), not affected by neurodegenerative disorders (controls). c) Proteins were extracted from post mortem material. Western blot analysis was performed with anti-OR51E1 antibody. Protein extract from SH-SY5Y cells was included as negative control. β-actin was used as loading control. Images are representative of n = 2 independent experiment with n = 2 independent SN samples. d) ZENBU genome browser view of CAGE signals for OR2L13 and OR51E1 in human SN. Genomic coordinates are shown on top and scale of expression values (TPM) on the left. Black arrows in each track highlight transcription start sites (TSS). At the bottom, thick arrowheads indicate direction of transcription. e) qRT-PCR validation of human mDA-ORs expression in human tissues. A panel of human RNAs from various tissues was used to validate atypical expression of OR2L13 and OR51E1. Brain was arbitrarily set as 1. f) qRT-PCR of SN from control and PD post mortem brain samples. *p < 0.05; **p < 0.01; ***p < 0.001.