Figure 2

mRNA profiling of S. miltiorrhiza hairy roots by high-throughput sequencing. (A) Length distribution of the 25,793 identified isotigs. (B) Summary of automated annotation. Close homologs refers to isotigs with BLASTX hits that exhibit ≥ 30% identity over ≥ 30% of the sequence, distal homologs refer to isotigs whose BLASTX hits do not met this criteria, while no hits refer to isotigs without any BLASTX hits. (C) Venn diagram showing the number (and the corresponding percentage of the total) of genes expressed at the various time points. (D) Venn diagram showing the number of differentially expressed (DE) genes found at the various time points.