Figure 2From: Rapid transcriptome sequencing of an invasive pest, the brown marmorated stink bug Halyomorpha halysStrand-specific sequencing with DSN normalization. In strand-specific sequencing, the final sequencing reads can be assigned to a particular strand of DNA. This is accomplished by a second-strand synthesis with dUTP, ligation of adaptors to double-stranded DNA, and degradation of the second strand with UNG. The result is a fragment to be sequenced that is differentially labeled on the two ends in a manner that dictates how they are loaded on the sequencer and thus the order in which the ends are sequenced.Back to article page