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Figure 2 | BMC Genomics

Figure 2

From: Rapid transcriptome sequencing of an invasive pest, the brown marmorated stink bug Halyomorpha halys

Figure 2

Strand-specific sequencing with DSN normalization. In strand-specific sequencing, the final sequencing reads can be assigned to a particular strand of DNA. This is accomplished by a second-strand synthesis with dUTP, ligation of adaptors to double-stranded DNA, and degradation of the second strand with UNG. The result is a fragment to be sequenced that is differentially labeled on the two ends in a manner that dictates how they are loaded on the sequencer and thus the order in which the ends are sequenced.

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