Figure 8

Lysozyme phylogeny. Homologues to the two putative lysozymes from BMSB were identified using a BLASTX [35] search of NR and the NCBI whole transcriptome database, aligned with MAFFT [40], and maximum likelihood phylogenies generated with RAxML [37]. The two lysozymes cluster with lysozymes from other Hemiptera as well as α-Proteobacteria. This cluster includes the lysozymes that have previously been attributed to lateral gene transfer from bacteria in the order Rickettsiales, which includes Wolbachia endosymbionts of arthropods. Presence in multiple hemipteran lineages may suggest that this transfer predates the divergence of all Hemiptera. Outside the eukaryotic and Wolbachia clades, only the genus is provided, in order to aid visualization. Sequences taken from whole transcriptome assemblies are denoted with asterisks since it is important to carefully consider that the sequences may have arisen from contamination from the microbiome. The inset in the lower left corner illustrates the phylogeny of the suborders of Hemiptera [41].