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Figure 9 | BMC Genomics

Figure 9

From: Rapid transcriptome sequencing of an invasive pest, the brown marmorated stink bug Halyomorpha halys

Figure 9

Heat map of qRT-PCR data. All ten individual RNA samples were subjected to qRT-PCR for five insect housekeeping genes and nine genes with best hits to bacterial genes using BLAST. Within each sample the ∆Ct is illustrated in a heat map and was calculated as the difference between the average Ct across the four constitutively expressed genes (ThRS, L30, Tubulin, and L27) and the Ct value for the gene being interrogated. In this way the color coding represents the difference in expression between the gene queried and the average constitutively expressed housekeeping gene, which all had similar abundance levels. In most samples, comp18511_c0_seq1, comp549_c15_seq3, comp2753_c5_seq1, comp7015_c0_seq1, and comp11444_c0_seq1 were poorly expressed relative to the constitutively expressed genes. However, in fifth instar nymphs (dark green), comp18511_c0_seq1 was transcribed at levels similar to the constitutively expressed genes while in active adult females, comp549_c15_seq3 and comp2753_c5_seq1 were transcribed at levels 64-fold higher than the average constitutively expressed gene. Both bLysB, the putative amylase, and the putative BMSB mannanase transcripts were transcribed constitutively at levels similar to the other constitutively expressed genes. For RNA that did not amplify a Ct value was assigned of 43, which was the lowest Ct value measured across the dataset. This reflects the low abundance of a transcript that did not amplify, but still allowed for clustering. The value for comp11444_c0_seq1 in the 4th instar larvae replicate 3 is missing due to an aberrant amplification curve.

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