Table 1 Primer sequences used for this study
Marker | Sequences 5′to 3′ | Note |
|---|---|---|
M-73000 | F:ATTCCCACCCTTGATGATGT | Screening recombinant individuals |
R:TTCTTTAGCCAACTCCTTGC | Restriction enzyme: Taq I | |
M-137 | F:TAGCTTGGGATCGACATCTT | Screening recombinant individuals |
R:CTCGTTCTCGCATTCATTTA | Restriction enzyme: Mbo I | |
I2 | F:TGTGCTAAGTGAYGCASAGA | Amplification of I2 homologs |
R:TAGAGAGGGRAGRGCA | ||
T-I2-1 | F:GCTTGAATTTAGAGATATGCG | Mapping I2 homologs |
R:GCTTAGGGCAATAGAAGATAGT | ||
T-I2-5 | F:CCCTTCTACAAATTGGAGCACA | Mapping I2 homologs |
R:GGTAGTTTGCAGTATGCTAACA | ||
T-I2-8 | F:ACCACTGACTGTTACTTTCTAT R:ATTAGGGCAATGGTAGATCAC | Mapping I2 homologs |
T-I2-2 | F:TTCTCCTGTCATCTTGTTGTTC | Mapping I2 homologs |
R:CCAAGTAACGTTCTGGCAATTT | ||
T-I2-3 | F:GGCTTAATGGGCTTCGAGTC R:GTTCCACAAGTGACGGTAT | Mapping I2 homologs |
T-I2-6 | F:AGATTGAAAGCATTAAATATCA | Mapping I2 homologs |
R:ATTGGATACCTCAAGTCTTGT | ||
T-I2-7 | F:GTGGCTTAATGAGCTTCGAGA | Mapping I2 homologs |
R:TCACATTCTACCACTTTCAAG | ||
T-I2-9 | F:CCAATCCAGTTCACTCTTTCA | Mapping I2 homologs |
R:ACCAGTTTCTTGCAATCATA | ||
T-I2-10 | F:GTCCCAAATCCTTCAGAG | Mapping I2 homologs |
R:CAAAGATTTTCAGGCAACTTT | ||
Race-P1 | R: GGAAGATCATTGTAGCTCAACATYA | Identification of miRNAs’ cleavage sites |
Race-P2 | R: CGTGTMGTCACAATGATCTTACTTCC | Identification of miRNAs’ cleavage sites |
Race-P3 | R: TTTCCTTCAATTTGACTTGWAGC | Identification of miRNAs’ cleavage sites |