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Table 1 Statistics of DGE sequencing at the three developmental stages

From: Transcriptome differences in the hypopharyngeal gland between Western Honeybees (Apis mellifera) and Eastern Honeybees (Apis cerana)

Summary   NEW 1 NEW 2 Nurse 1 Nurse 2 Forager 1 Forager 2 Total
Raw tag Aml 6,000,000 6,000,000 6,000,000 6,000,000 9,020,722 9,018,194 42,038,916
Acc 6,000,000 6,000,000 6,000,000 6,000,000 6,000,000 6,000,000 36,000,000
Distinct tag Aml 211,272 198,230 157,958 160,974 289,909 256,732 1,275,075
Acc 215,863 219,554 144,442 130,883 170,217 213,917 1,094,876
Clean tag Aml 5,882,059(98.03%) 5,890,202(98.17%) 5,910,901(98.52%) 5,911,105(98.52%) 5,423,816(60.13%) 5,849,152(64.86%) 34867235(82.94%)
Acc 5,883,978(98.07%) 5,878,500(97.98%) 5,917,830(98.63%) 5,923,277(98.72%) 5,904,132(98.40%) 5,871,384(97.86%) 35,379,101(98.28%)
Distinct clean tag Aml 100,676(47.65%) 95,104(47.98%) 77,435(49.02%) 80,286(49.88%) 61,548(21.23%) 52,987(20.64%) 468,036(36.71%)
Acc 106,580(49.37%) 104,133(47.43%) 71,482(49.49%) 63,646(48.63%) 82,699(48.58%) 95,883(44.82%) 524,423(47.90%)
All tag mapping to gene Aml 3,958,194(67.29%) 4,039,673(68.58%) 4,749,009(80.34%) 4,687,158(79.29%) 4,578,511(84.41%) 5,206,584(89.01%) 27,219,129(78.07%)
Acc 2,476,714(42.09%) 2,570,588(43.73%) 2,740,538(46.31%) 2,755,741(46.52%) 2,422,568(41.03%) 3,091,826(52.66%) 16,057,975(45.39%)
Unambiguous tag mapping to gene Aml 3,549,912(60.35%) 3,579,074(60.76%) 3,550,900(60.07%) 3,551,385(60.08%) 4,465,416(82.33%) 5,157,312(88.17%) 23,853,999(68.41%)
Acc 2,370,309(40.28%) 2,512,797(42.75%) 2,520,841(42.60%) 2,321,965(39.20%) 2,404,037(40.72%) 3,014,975(51.35%) 15,144,924(42.81%)
Mapping to genome Aml 1,100,732(18.71%) 1,025,724(17.41%) 732,560(12.39%) 751,100(12.71%) 558,499(10.30%) 458,366(7.84%) 4,626,981(13.27%)
Acc 925,681(15.73%) 1,002,791(17.06%) 659,768(11.15%) 492,653(8.32%) 923,103(15.63%) 773,385(13.17%) 4,777,381(13.50%)
All tag-mapped genes Aml 8,060(72.88%) 7,705(69.67%) 7,725(69.85%) 7,464(67.49%) 7,388(66.8%) 6,761(61.13%) 45,103(67.97%)
Acc 7,317(66.16%) 7,385(66.77%) 6,656(60.18%) 6,450(58.32%) 6,961(62.94%) 7,045(63.7%) 41,814(63.01%)
Unambiguous tag-mapped genes Aml 7,826(70.76%) 7,491(67.73%) 7,503(67.84%) 7,255(65.6%) 7,184(64.95%) 6,578(59.48%) 43,837(66.06%)
Acc 7,077(63.99%) 7,157(64.71%) 6,469(58.49%) 6,250(56.51%) 6,743(60.97%) 6,824(61.7%) 40,520(61.06%)
Unknown tag Aml 823,133(13.99%) 824,805(14.00%) 429,332(7.26%) 472,847(8.00%) 286,806(5.29%) 184,202(3.15%) 3,021,125(8.66%)
  Acc 2,481,583(42.18%) 2,305,121(39.21%) 2,517,524(42.54%) 2,674,883(45.16%) 2,558,461(43.33%) 2,006,173(34.17%) 14,543,745(41.11%)