Table 2 Polymerase chain reaction (PCR) and sequencing primers used to amplify portions of the mtDNA and Y chromosome for phylogenetic analysis to verify Mauritius origin of the resequenced cynomolgus macaques from the Goswami et al.[28] study
From: Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius
| Gene | Primer Name | Sequence (5′ to 3′) |
|---|---|---|
| mtDNA | ||
| HSVI; partial cytb | ||
| Forward | CCG CCC ACT CAG CCA ATT CCT GTT CT | |
| Reverse | CCC GTG ATC CAT CGA GAT GTC TT | |
| Y chromosome | ||
| SRY | ||
| SW2 | CTT GAG AAT GAA TAC ATT GTC AGG G | |
| SW3B | AGG TCT TTG TAG CCA ATG TTA CCC G | |
| F1 | AGT GAA GCG ACC CAT GAA YG | |
| R1 | GTA TCC CAG MTG CTT GCT GAT C | |
| TSPY | ||
| TSPY-A | AGC CAG GAA GGC CTT TTC TCG | |
| TSPY5R | CTG TGC ATA AGA CCA TGC TGA G | |
| 470 F | CGG CAG TTC TCT GCA T | |
| E690R | TCG RCA TGG ATA AGA CGG AC |