Table 3 Primers used in this study
From: Transcriptomic dissection of the rice – Burkholderia glumae interaction
Gene | Primer name | Primer sequence (5′ → 3′) |
|---|---|---|
LOC_Os11g12340 | RPM1-L1-F (forward) | 5′-GAAGCTCTCAAACCAGAATCAGAGGAG-3′ |
RPM1-L1-R (reverse) | 5′-TATTCACCCTGTTAAGGCTAAGAAAGAC-3′ | |
LOC_Os11g12330 | RPM1-L2-F (forward) | 5′-AATCTCAAGCCAAGTTCGGAAAATCTC-3′ |
RPM1-L2-R (reverse) | 5′-CTCACCCTGTTAAGTTTAAGATAGGTG-3′ | |
LOC_Os11g12320 | RPM1-L3-F (forward) | 5′-CTTCGAGTGCTCGGGCTACATGTTCC-3′ |
RPM1-L3-R (reverse) | 5′-CTCTCTCCAGCCCTGCCAGTGAATCG-3′ | |
LOC_Os11g12040 | RPM1-L4-F (forward) | 5′-CACCAACGTATGTAAGCTCCCAGAATG-3′ |
RPM1-L4-R (reverse) | 5′-CTGGAGTTCTTCCAAACCTGATAACC-3′ | |
LOC_Os11g12300 | NBS-LRR2-F (forward) | 5′-AACAAGAATAGAAAAGGAACTGAAGATG-3′ |
NBS-LRR2-R (reverse) | 5′-CTCCTTTCACAAACTTCTTCATTTTCC-3′ | |
LOC_Os11g12000 | NBS-LRR3-F (forward) | 5′-AGTGAGCAAGGTAACACAGTGATAACG-3′ |
NBS-LRR3-R (reverse) | 5′-GTTCAGGGTACCCAATTGTTGGCGTC-3′ | |
LOC_Os08g28460 | NBS-LRR7-F (forward) | 5′-CCTTATGGCAACACTGAATCAGGGGAC-3′ |
NBS-LRR7-R (reverse) | 5′-ATGCACATGTGGGAAGCAGCAAGATTC-3′ | |
LOC_Os11g11550 | NBS-LRR8-F (forward) | 5′-GTCCCAAGTCCAAGCTGATATCAGGC-3′ |
NBS-LRR8-R (reverse) | 5′-GAGTTCTCATATTGTACACAGAACCGG-3′ | |
LOC_Os08g25050 | PIF-L-F (forward) | 5′-GAGACAATACAAGCTATGGAAGAGCC-3′ |
PIF-L-R (reverse) | 5′-CGCACAACATTGTACCTTCTTCGGTG-3′ | |
18S rRNA | 18S rRNA-1 (forward) | 5′-GGAATTGACGGAAGGGCACCACCAGGC-3′ |
18S rRNA-2 (reverse) | 5′-GGACATCTAAGGGCATCACAGACCTG-3′ |