Figure 3

Co-expression network analysis of bovine pre-implantation development. (A). Hierarchical cluster tree showing modules of co-expressed genes identified by WGCNA. A total of 17 co-expressed modules were found during bovine pre-implantation development and were represented by branches and labeled by different colors to the right of the tree. The height (X-axis) indicates levels of correlation. (B). Heatmap of correlations (and corresponding P-values) between co-expressed modules and stage of development. The color scheme, from blue through white to red, indicates the levels of correlation, from low to high. The stage-specific modules identified are highly correlated (i.e. over-expressed) with distinct developmental stages (columns). (C). Functional terms of stage-specific modules of co-expressed genes during bovine pre-implantation development. A systematic and sequential changes in functions of co-expressed genes were observed as the embryos progress through early development.