Figure 2

qPCR analysis of piRNAs and piRNA-linked non-coding gene. qPCR quantitative expression analysis of three repeat sequence-linked putative piRNAs that were upregulated in PGCs, along with CR1. cDNA templates used for the amplification of piRNAs and genes were prepared separately, and amplified using the appropriate piRNA- and gene-specific primers. The expression of piRNAs and CR1 were normalized against that of snoRNA and GAPDH, respectively. Bars indicate the SEM of triplicate analyses.