Differentially expressed hematological genes in RPS24 MO. (A) A total of 25 differentially expressed hematological genes (by fold-change >1.3 and p-value <0.05) were detected in RPS24-deficient zebrafish embryos in 48hpf. The expression intensity of these genes were normalized to (-1, 1) and represented as heat map by R script. (B) Representative experimental validation of the regulated genes by Real-time PCR analysis. Gene expression was represented as the mean ± SD and One-way ANOVA was performed for comparison between Control and RPS24-deficient zebrafish embryos (**P <0.001, *P <0.01, n = 3). Gene expression in Control samples was normalized to 1.