Figure 5
Quantitative RT-PCR validation of randomly selected unigenes. (A) qRT-PCR validation of the expression patterns of 11 representative unigenes from different comparisons. The y-axis represents qRT-PCR relative expression levels from three independent biological replicates (left x-axis) and the log2 fold-change of the unigene (right x-axis). (B) Correlation analysis of 26 highly differentially expressed unigenes in leaves under salt stress for 4 and 24 h based on qRT-PCR and mRNA-seq data; Pearson correlation coefficients (r) are 0.7937 and 0.8767, respectively (P < 0.001).