Expression of Cu-miRNA in plants inoculated with A. brasilense and H. seropedicae . Relative expression of miR397, miR398, miR408 and miR528 in response to inoculation with A. brasilense and H. seropedicae. Biological replicates were analyzed (Experiment A and B). The error bars represent the standard deviation between three technical replicates. *represent significant changes in miRNA expression between control and inoculated samples for each experiment (p-value < 0.05).