Figure 5
From: Maize protein phosphatase gene family: identification and molecular characterization
Differential gene expression and real-time PCR analysis of ZmPP genes. A Effect of PAO on the key enzymes of ABA biosynthesis under water stress condition in Arabidopsis. 1, control; 2, PAO-treated; 3, negative control. B 1, stable expression of ZmPP1-GUS fusion protein in Arabidopsis chloroplast; 2, transient expression of ZmPP1-GFP fusion protein in onion epidermal cell; 3, transient expression of GFP protein only. C Analysis of gene expression in Arabidopsis transformed with estradiol-inducible ZmPP1. 1, wild-ethanol treated; 2, wild-estradiol treated; 3, ZmPP1 over-expression1 (ZmPP1OE1)-estradiol treated; ZmPP1OE2-estradiol treated. D Expression profiles of ZmPP genes under moderate drought stress (M/C), severe drought stress (S/C), and re-watering (R/C) as compared to control seedlings in Han21 and Ye478, respectively. E Real-time PCR analysis of 24 differently expressed PP genes under drought stress. F ABA biosynthesis and ABA-dependent pathway of response to drought in plants. The red arrows represent expression levels only detected by RNA-seq under drought stress.