Identification of male-specific amh duplication, sexually differentially expressed genes and microRNAs at early embryonic development of Nile tilapia (Oreochromis niloticus)

Eshel, Orly; Shirak, Andrey; Dor, Lior; Band, Mark; Zak, Tatyana; Markovich-Gordon, Michal; Chalifa-Caspi, Vered; Feldmesser, Esther; Weller, Joel I; Seroussi, Eyal; Hulata, Gideon; Ron, Micha

doi:10.1186/1471-2164-15-774

Table 1 Primers for gene expression validation with qPCR and amh PCR and sequence analysis

From: Identification of male-specific amh duplication, sexually differentially expressed genes and microRNAs at early embryonic development of Nile tilapia (Oreochromis niloticus)

Gene symbol/probe	Description	Accession number	Forward and reverse primers (from 5’ to 3’)
tspan8	tetraspanin-8	XM_003448079	TGTATGTTGTGGAATAGGCATCA
			GGTGATTTGTAAAGCTGTTTCG
fcgrt	major histocompatibility complex class I-related gene protein-like	ENSONIG00000008406	TGGTGTGAGCAAGGACTTCAT
			ACATGAACCAAAGGACTGTAAACT
cr/20β-hsd	carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase	XM_005473633	CCAAAATTGTTCGTTTTATTCTCG
			TTTCATTTTGATGCGTTCCA
gpa33	cell surface A33 antigen	XM_005466820	AATGTCCAAAAGCCAACCTAAA
			TACTACATCTGCACCTCGGAGA
rtn4Ip11	reticulon-4-interacting protein 1 homolog, mitochondrial	XM_003459954	GCATGTCAAGGCATCAAATAAA
			CCTCTGCTGGTTGTAAATGTGA
26098	hypothetical protein		TTCCTGAAGACAGTACAGTACAAAA
			CGAATTCTTCTGGTCAAGTTCTTC
zp3	zona pellucida sperm-binding protein 3 receptor-like	XM_005448435	TGTCTGTAACTACTCATTTGGATCA
			CCATTTTACAGATCCAACTTTCC
gapdh	glyceraldehyde-3-phosphate dehydrogenase	XM_003452690	GGCATCGTGGAAGGTCTCAT
			CATTTTACCAGAGGGCCCGT
rpp30	ribonuclease P protein subunit p30	XM_005471600	CCCGACTCCTATCAACGAAC
			AAAGTGACTCGCGTCTGACA
amh	anti-Müllerian hormone¹	DQ257619.1	TTCTTATCGCTCCGACTTCTTC
			TAGGGCTGGTTGATATGGAATC
	anti-Müllerian hormone - exon VII²	XM_003451305	AGCAGCTCTAGCGGCATCCACA
			TGTGTTTTCTTTCTGCGTCCGCCA
	1³: 5’ UTR, exon I, intron 1, exon II	ENSONIG00000004781	AGAGGAGTCATCAGTCCAAAGC
			AGATGTCCTCCACGAAGCAT
	2: exon II, intron II, exon III	ENSONIG00000004781	AAGACCCCATCATCACCATC
			TTGTCTGAGCCGTAATCTGC
	3: exon IV, intron III, exon V, intron IV, exon VI	ENSONIG00000004781	GGAAAATCATCAGAGGGGAGT
			CTGCCGACTTCAGAACTTTT
	4: intron VI, exon VII, 3’ UTR	ENSONIG00000004781	CGGTCCCAGTGACCTATGAG
			AAGTACACGTGGTGTATTGTAATTGA
	5: 3’ UTR	ENSONIG00000004781	CCCCAGCATTTATAACTTTCACA
			CCTGCCTCAAGTATGCCTTT
	LM⁴: intron VI, exon VII	ENSONIG00000004781	TGTGTTTTCTTTCTGCGTCCGCCA
			AGCAGCTCTAGCGGCATCCACA

¹qPCR validation of microarray.
²PCR of cDNA.
³set of 5 primer pairs for sequencing.
⁴Linkage mapping.

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ISSN: 1471-2164

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