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Table 2 Gene expression validation of microarray results

From: Identification of male-specific amh duplication, sexually differentially expressed genes and microRNAs at early embryonic development of Nile tilapia (Oreochromis niloticus)

 

qPCR

Over expressed in

Gene symbol

Microarray

cDNA

Multiple Tm peaks3

gDNA

  

FC1

p-value

FC

p-value

 

FC

p-value

Male

amh

0.5

6 × 10−9

0.27

0.002

 

0.75

7.4 × 10−4

 

tspan8

0.2

4.4 × 10−16

0.0001

5 × 10−11

 

0.0013

1.2 × 10−4

 

fcgrt

0.5

6.5 × 10−8

ND2

 

√

ND

 

Female

cr/20β-hsd

3.5

1.0 × 10−17

352

2.8 × 10−11

√

669

0.002

 

gpa33

3.4

6.0 × 10−9

88.7

2.6 × 10−5

√

15.8

3.3 × 10−6

 

rtn4Ip1

2.5

1.0 × 10−17

2.1

0.0001

 

ND

 
 

CUST_26098

4.2

1.6 × 10−7

4.46

0.004

 

ND

 
 

zp3

2.6

3.0 × 10−9

2

0.004

 

ND

 
  1. Mean ratio of female to male gene expression by qPCR from cDNA of embryos from 2 to 9 dpf is presented. The qPCR analysis of genomic DNA (gDNA) was applied for indication of copy number variation.
  2. 1FC: fold change of female to male expression: LS means for Microarray and means for qPCR based on cDNA of embryos from 2 to 9 dpf.
  3. 2ND: not determined.
  4. 3Multiple TM peaks resulting from melting curve analysis.
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BMC Genomics

ISSN: 1471-2164

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