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Table 2 Gene expression validation of microarray results

From: Identification of male-specific amh duplication, sexually differentially expressed genes and microRNAs at early embryonic development of Nile tilapia (Oreochromis niloticus)

  qPCR
Over expressed in Gene symbol Microarray cDNA Multiple Tm peaks3 gDNA
   FC1 p-value FC p-value   FC p-value
Male amh 0.5 6 × 10−9 0.27 0.002   0.75 7.4 × 10−4
  tspan8 0.2 4.4 × 10−16 0.0001 5 × 10−11   0.0013 1.2 × 10−4
  fcgrt 0.5 6.5 × 10−8 ND2   ND  
Female cr/20β-hsd 3.5 1.0 × 10−17 352 2.8 × 10−11 669 0.002
  gpa33 3.4 6.0 × 10−9 88.7 2.6 × 10−5 15.8 3.3 × 10−6
  rtn4Ip1 2.5 1.0 × 10−17 2.1 0.0001   ND  
  CUST_26098 4.2 1.6 × 10−7 4.46 0.004   ND  
  zp3 2.6 3.0 × 10−9 2 0.004   ND  
  1. Mean ratio of female to male gene expression by qPCR from cDNA of embryos from 2 to 9 dpf is presented. The qPCR analysis of genomic DNA (gDNA) was applied for indication of copy number variation.
  2. 1FC: fold change of female to male expression: LS means for Microarray and means for qPCR based on cDNA of embryos from 2 to 9 dpf.
  3. 2ND: not determined.
  4. 3Multiple TM peaks resulting from melting curve analysis.