Figure 7
From: Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins
Domain analysis and phylogenetic tree of DEAD RNA helicase domain proteins. (A) Conserved Domains Database search shows both Dbp45A and Vasa RNA helicases has DEAD domain and a C-terminal Helicase domain while DDX18/Has1 subfamily of helicase has an unknown domain which is present in all concerned helicase proteins of this family indicating clear orthology. (B) The neighbour-joining tree is based on the alignment of the conserved DEAD domain containing RNA helicases. The DEAD helicases forms three distinct clusters categorized into three subfamilies Dbp45A, Vasa and DDX18/Has1. Sf-DDX18 like proteins is closest with Bombyx mori but similar with Pitchoune of Drosophila and Tribolium too. Vasa subgroup family of proteins being important for a piRNA pathway suggests Sf-Vasa might participate in Piwi associated RNA silencing.