Figure 5

12p13 deletion-associated gene dosage effect. A: Comparison of CK-AML with (n = 4) and without 12p13 deletion (n = 28). The box plot shows the expression values for the genes located in the critical 12p region comprising the following genes: ETV6, BCL2L14, LRP6, DUSP16, CDKN1B and GPRC5D (normalized log2 transformed gene expression values). Cases with a 12p13 deletion show a significantly lower expression level for the deleted genes (p < 0.001, unpaired t-test). B: Comparison of the clones located within the critical region and clones located in unaltered chromosome 12 regions shows a significantly lower expression for the 12p critical region clones in AML cases with a 12p13 deletion (p = 0.041, ANOVA one-way analysis of variance). C: Comparison of CK-AML cases with (n = 8) and without 12p13 deletion (n = 8). The box plot shows the RPKM values of RNAseq data representing the expression level of the genes ETV6, BCL2L14, LRP6, DUSP16, CDKN1B and GPRC5D. Cases with a 12p13 deletion show a significantly lower expression level for ETV6 (p < 0.03, unpaired t-test) and CDKN1B (p < 0.003, unpaired t-test). BCL2L14, LRP6, DUSP16 and GPRC5D show no or very low expression levels. All data represented here were obtained from the TCGA network (http://cancergenome.nih.gov).