Figure 1

The experimental flow of genome-wide CNV analyses in miPSCs using high-density CGH microarrays. A total of 41 miPSC lines were derived from the same donor of MEF B2 using different reprogramming factors and/or dosages. Genomic DNAs of the parental MEF B2 and various miPSC lines were respectively extracted and fragmented. Cy5-dUTP was used for labeling miPSC DNAs and Cy3-dUTP for the donor DNAs. Each labeled miPSC DNA was hybridized together with a labeled donor DNA onto the mouse genome CGH microarrays. Microarray handling and data analysis were conducted following the Agilent oligonucleotide CGH protocol. Examples were shown for a copy number loss (deletion; depicted by green concave) and a copy number gain (duplication; depicted by red convex) at the bottom.