Figure 5

Origin architecture is remodelled during activation. Average nucleosomal profiles from early (A) and late (B) origin groups in G1 (blue line) and S-phase (green line). (C), Nucleosome patterns of the early origins ARS 305 (top panel) and ARS 731 (bottom panel) during G1 (blue) and S-phase (green). Genes, replication origins and ACS are represented as in Figure 3. Dotted lines indicate the center of the nucleosomes flanking the origins during G1. Nucleosome remodelling at early origins is summarized in a schematic diagram where grey ovals represent nucleosomes in S-phase and the dotted nucleosome indicates the position of the +1 N (the first nucleosome downstream of the ACS) in G1. Nucleosome displacement is represented by a black arrow. (D), As in C but for the late origins ARS 603 and ARS 501. (E), Neutral/neutral 2D gel analysis was performed to analyze ARS305 and ARS603 activity. Genomic DNA was prepared from cells released from α-factor arrest into YPD + 0.2 M HU and collected at 15, 30, 45 and 60 min.