Figure 2

Comparison of qPCR results with the microarray results for eight differentially expressed genes. The expression levels for eight differentially expressed genes were determined by quantitative polymerase chain reaction (qPCR) with the same RNA pools that were used for the microarray. These include the following differentially expressed genes: galanin peptide (GALP), glutamate decarboxylase 1 (GAD1), corticotropin releasing factor binding protein (CRFBP), C-type natriuretic peptide (CNP), gamma-aminobutyric acid receptor subunit δ (GABRD), neuropeptide Y (NPY), mammalian ependymin-related protein (MERP) and the iroquois-class homeodomain protein 2 (IRX2). Bars represent mean fold changes, error bars represent standard deviations and * represents significant (P < 0.05) increases in the level of expression in the brains of sexually mature male eels in comparison to immature male eels.